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doi: 10.3390/toxins13070485

The inclusion body was dissolved in 8 M urea, 30 mM Tris-HCl pH 8.0, 1 mM EDTA and 5 mM DTT and refolded in buffer containing 0.5 M L-Arginine pH 8.0, 0.6 mM oxidized glutathione overnight at room temperature