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NFE2L2 mRNA levels stay constant after exposure to DEM, indicating that Nrf2 is not positively regulating itself by expression of NFE2L2 mRNA

User experimentation suggests that longer initial treatment periods (6+ months continuous) support better benefit retention during subsequent breaks, complete discontinuation breaks of 1-2 months show acceptable benefit maintenance for most users, and very extended breaks (3-6+ months) increase risk of regression toward pre-treatment baseline particularly for short initial treatment courses