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(General reference on nasal bioavailability and molecular weight thresholds.) Merkus FW, Verhoef JC, Schipper NG, Marttin E

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Conclusion Nasal spray testing encompasses a uniquely demanding suite of analytical requirements that extends far beyond standard pharmaceutical testing encompassing device performance characterization (spray pattern, plume geometry, droplet size distribution, dose uniformity, priming) alongside conventional formulation quality tests (assay, pH, osmolality, viscosity, microbial safety, stability) and container-closure system evaluation (leachables/extractables, container closure integrity)