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To interrogate whether melatonin could restrain ILC2 effector function we employed an adoptive transfer model, whereby activated ILC2s were isolated from Rag2 -/- mice, cultured for 24h in the presence or absence of melatonin, and then adoptively transferred the ILC2s into Rag2 -/- GC -/- mice utilizing established protocol as described by our group previously (48, 49) ( Figure 4A )

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These proteins are involved in the Ras/Raf/MEK/ERK signaling pathway which plays a role in cellular proliferation and survival and is involved in the pathophysiology of UF

Such studies have shown that antibiotic administration might increase the effectiveness of L-DOPA therapy, indicating that gut microorganisms affect metabolism and the effectiveness of the drug (Hashim et al., 2014)