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Methods Construct modification and expression of full-length GLP-1R The optimized gene of full-length GLP-1R was cloned into the modified pFastbac1 vector at the BamHI/HindIII sites and the native signal sequence was replaced by haemagglutinin (HA) to enhance receptor expression (see primers in Supplementary Table 2), followed by a flag-tag, a 10 His-tag and a tobacco etch virus protease (TEV) site at the N-terminus

However, Section 503A explicitly states that neither licensed pharmacists nor physicians can make copies when the medications are commercially available

In vivo skin penetration assay Female BALB/c nude mice with an average weight of 20 g were purchased from SLAC Laboratory Animal Co., Ltd