semaglutide recombinant synthesis Total Synthesis of Semaglutide Based on a Soluble Hydrophobic-Support-Assisted Liquid-Phase Synthetic Method Commercial manufacturing of current good

semaglutide recombinant synthesis

semaglutide recombinant synthesis Total Synthesis of Semaglutide Based on a Soluble Hydrophobic-Support-Assisted Liquid-Phase Synthetic Method Commercial manufacturing of current good

Name: semaglutide recombinant synthesis Total Synthesis of Semaglutide Based on a Soluble Hydrophobic-Support-Assisted Liquid-Phase Synthetic Method Commercial manufacturing of current good
Brand: wefit.es
SKU: 46558913732
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SKU: 46558913732

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Description

Cooled the compound to 5-10C and acidified by using IN HC1 solution and stirred for 15 min at same temperature

Wash buffer : 50mM Tris-HCl 20mM Immidazole, pH 8.0 0.1

DIPEA (8.82 ml) was slowly added to the reaction mixture at 15-20C and stirred for 5 hr at same temperature

The desired monocistronic (single copy) of His6-AV-Ubiquitin- semapeptide (nucleotide sequence)-encoding gene PCR product of ~320bp was obtained as evident by 1% (w/v) Agarose gel electrophoresis (Figure 1)