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An in vitro study found that prolonged oven heating or boiling (i.e., six minutes or longer) suppressed the inhibitory effect of uncrushed and crushed garlic on platelet aggregation, but crushed garlic retained more anti-aggregatory activity compared to uncrushed garlic (136)

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For transwell migration assays, transwell chambers were inserted into the wells of 24-well cell plates, and RKO and DLD-1cells resuspended with the FBS-free medium were added into the upper room