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More Identification and expression of semaglutide main chain P29 with SNAC and DDDDK tags

[0068] Before the coupling of the Lys side chain, Alloc protecting groups were removed twice with a solution of 0.2 equivalents of Pd(PPh 3 ) 4 and 10 equivalents of morpholine in methylene chloride for 30 min each time

et la chane latrale de Lys 26 est prpare par synthse fragmentaire, et un fragment entirement protg de Fmoc-PEG-PEG--Glu-acide octadcanedioque utilis dans la synthse fragmentaire de la chane latrale de Lys 26 est prpar par le couplage successif d'une rsine de chlorure du Fmoc-PEG-OH, du Fmoc-PEG-OH, du Fmoc-Glu(OH)-OtBu et un ester mono-tert-buytlique d'acide octadcanedioque, clivage et recristallisation pour obtenir un PEG-PEG--Glu-ester mono-tert-butylique d'acide acide octadcanedioque

DCHA and Boc-His (Trt)-OH are used as raw materials in the procedures, and accordingly His racemization risks can be reduced to the greatest extent