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Histological analysis reveals that aged kidneys accumulate senescent cells primarily in tubular regions, identified by loss of Lamin B1 (LMNB1), elevated senescence-associated -galactosidase activity, and expression of inflammatory IL-6

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GST Tag Interference with Protein Function Challenge : In some cases, the GST tag can interfere with the function of the target protein, particularly if the protein's active site or interaction domains are close to the fusion point

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