glutathione and mitochondrial function (GSH), a powerful intracellular antioxidant, plays a Controlling glutathione entry into mitochondria:

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One reason could be that phase II metabolism occurring in vivo leads to a decrease in the antioxidant capacity of metabolites (Reference Pollard, Kuhnle and Vauzour75)

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Determination of reactive oxygen species (ROS) using dihydroethidium (DHE) ROS was determined using fixed tissues following a previously reported protocol (Lee et al ., 2024)