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According to the method, the compound having a structure represented as the formula I is taken as an intermediate and coupling of each amino acid or amino acid derivative step by step through solid-phase synthesis is carried out, thereby improving the yield of semaglutide

Example 5: Isoelectric Point (Pi) Precipitation of Semapeptide Semapeptide present in cleavage sample was subjected to isoelectric point (pl) precipitation

2) Liquor Ammonium (12.5%) was prepared in RO water of about 1500ml

- Use appropriate reaction conditions and reducing agents (such as triethylamine-trimercaptopropane) to promote the formation of disulfide bonds between cysteine and other amino acids