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3 Mechanisms of action of natural supplements against breast cancer 3.1 Anti-inflammatory and antioxidant pathways The chronic inflammatory microenvironment in the breast activates nuclear factor-B (NF-B), cyclooxygenase-2 (COX-2), signalling transducers and activators of transcription-3 (STAT3), activator protein-1, and mitogen-activated protein kinase signalling cascades

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Add the elution buffer to the column, cap both ends, and incubate for a few minutes before uncapping and dripping your elution out of the bottom (Figure 4)