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Briefly, 270 L model media (potato wastewater with glycerol) and experimental media (with the addition of Se 4+ at a concentration of 1060 mg/L) and 30 L of yeast from an inoculum culture were introduced to all wells of the Bioscreen C apparatus plate (Oy Growth Curves Ab Ltd., Finland)

Cultures were allowed to equilibrate in a recording chamber mounted on inverted microscope (IX-71

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This means molecules move faster and collide more frequently, which accelerates the rate of all chemical reactions