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Doxorubicin stock solution (10 mM, 100% DMSO) was serially diluted in unbuffered seahorse assay medium to prepare 6 concentrations: 400, 300, 200, 100, 80 and 40 M

This means you might feel effects sooner, and peak concentrations may be higher, which could influence sleep architecture (such as deeper slow wave sleep) or support stressful recovery periods

Drug interactions: Certain medications, such as omeprazole, lansoprazole, cimetidine, and metformin, can lead to low vitamin B12 levels

Amino acid concentrations were quantified using Skyline software (MacCoss Lab Software), where MRM transitions (ion pairs) were automatically identified and integrated with manual verification as required