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The GST-PRMT1 fusion protein or GST protein was mixed with His-UBE2m fusion protein overnight under gentle rotation at 4 C and then incubated with GST beads (78602, Thermo Fisher Scientific) at 4 C for 3 h

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6 Limitations and future directions The above studies discussed the effects of SSRIs in the reproductive system, but the following limitations exist: the small sample sizes of several studies and the lack of large-scale, multicenter, randomized controlled trials