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Sleeping pills (benzodiazepines, Z-drugs) suppress cortical activity and actually reduce deep sleep despite increasing total sleep time

While research is still emerging, early studies suggest benefits for brain health, physical performance, and even longevity

Other neurotransmitters and neuropeptides can also modulate prolactin secretion, as endothelin, TGF beta1, angiotensin, somatostatin, substance P, neurotensin, calcitonin, EGF, natriuretic atrial peptide, bombesin, cholecystokinin, acetylcholine, and vasopressin

Additionally, the time required to complete the enzymatic activity measurements was prolonged, and the method needed to be more easily adaptable to microplate readers due to the necessity of protein precipitation