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Refinement was performed using Phenix 54 and Refmac5 55,56 , coupled with rounds of manual model building, real-space refinement and structure validation performed using COOT 57
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Importantly, this proof-of-concept study showed that the relative abundance of Lactobacillus , a genus associated with weight loss 58 , was uniquely increased and Erysipelatoclostridium was distinctly decreased in mice given 5A-1MQ treatment and an LD switch compared to mice switched to LD and given vehicle treatment
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