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The peptides were loaded onto a reverse phase trap column (Thermo Scientific Acclaim PepMap100, 100 m*2 cm, nanoViper C18) connected to the C18-reversed-phase analytical column (Thermo Scientific Easy Column) in buffer A (0.1% Formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% Formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology

When gastric emptying is slowed, that gas has nowhere to go, leading to painful bloating and sulfur burps. Similarly, highly acidic foods like citrus fruits or spicy peppers can irritate the stomach lining

MGCA1 can be distinguished from the other forms of MGCA because the levels of 3-methylglutaconic acid are highly elevated, whereas levels of methylglutaric acid are usually only slightly elevated, and there is a high level of 3-hydroxyisovaleric acid excretion which is not seen in the other forms of MGCA