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The pellet was subsequently washed with 10 ml of the G-solution, which comprised of 500 ml HBSS (BE10-508F, Life Technologies) with 10% BSA (126615, Sigma Aldrich) and 1% Pen-Strep (15140122, Life Technologies), and re-suspended in 5.5 ml of gradient solution (15% Optiprep (5 ml 10% RPMI, Life Technologies) + 3 ml of 40% Optiprep, which was diluted from 60 % Optiprep with G-solution (D1556, Sigma Aldrich)) per sample, and placed on top of 2.5 ml of the gradient solution

Blood donation should be postponed if: Blood sugar is poorly controlled There has been a recent hospitalisation due to diabetes There is a fever, infection, or illness Severe anaemia is present There is advanced kidney, heart, or nerve damage Temporary deferral is done purely for safety and does not mean permanent ineligibility

They also recruited 45 demographically matched healthy controls

Youre comfortable with self-injections