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The GST fusion tag has been widely utilized to enhance protein solubility and protect the protein from proteolytic degradation while achieving native protein folding

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These observations are consistent with a growing body of literature demonstrating that early-life conditions can durably alter the methylation status of candidate genes in brain regions critically involved in stress signaling and transmission ( PI3K signaling is crucial for follicle activation, granulosa cell survival, and the preservation of ovarian reserve ( Figure 9D ), but demonstrated a substantial reduction in F2 high-dose offspring compared to both control and low-dose groups ( Figure 9E )