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Table 2 Oligonucleotides used as primers for quantification of different GPX4 mRNA isoforms and for mapping 5 end of I- GPX4 mRNA in SHSY-5Y cells

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Immunostaining was performed by seeding cells on glass coverslips, fixing with paraformaldehyde, permeabilizing with Triton X-100, and then incubating with primary anti-NF-B p65 antibody, followed by Alexa Fluor 546-conjugated secondary antibody

This low variation confirms that the modified electrode delivers a stable and consistent electrochemical response, demonstrating its suitability and reliability for practical applications such as pharmaceutical analysis and quality control