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Validation of differentially expressed proteins or biomarkers by peptide intensity and ELISA assay quantification To validate the differentially expressed proteins in plasma samples among the three groups, proteins (CST3, GAPDH, PCSK9, C1q, CBPB2, and PRXD6) with significant fold changes and pathway enrichment analysis scores were selected

PA most likely prevented activation of the pro-inflammatory factors and reduced LPS/D-Gal-induced liver injury by enhancing the zinc finger E-box-binding homeobox 2 expression level, and consequently, blocking activation of the NF-B signaling pathway in the liver (Yang et al., 2021a)

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To summarize briefly, Figure 2b clearly demonstrated that the level of HGF nitration was drastically decreased by LASSS-pretreatment as shown by Western blotting visualized by anti-nitroY mAb-positive bands normalized by total HGF -chain (third row lanes 2 and 3 and fourth row lanes 2 and 3 )