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Immunoblots of the leaf extracts confirmed a lower content of GSAAT and some other TBS proteins (such as PORB, GluTR and uroporphyrinogen decarboxylase (UROD)) in the ntrc/trxf-1 double mutant relative to wild-type seedlings ( Figures 4E, F ), and also revealed a slight decrease in accumulation of GSAAT, as previously reported ( ntrc, trxf1 and ntrc/trxf 1, respectively, compared to WT, as quantified in three different immunoblot experiments ( Figure 4F )