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Tobacco knockdown and overexpressor DHAR lines showed a substantial decrease and increase in extractable foliar activity, respectively (Chen and Gallie, DHAR2 increased the reduction state of the ascorbate pool, an effect that was observed both in control conditions and in response to aluminum stress (Yin et al., 2010)

10.1016/j.theriogenology.2022.08.030 79 SaN

Samples were grounded in solution comprising of thiobarbituric acid (TBA 0.25%) prepared in trichloroacetic acid (TCA 10%) followed by heating them at 95 C then cooled on ice and centrifuged for 10 min at 10,000 g