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Recombinant Sec TGR Expression and Purification A bacterial-type SECIS element was fused to the TGR open reading frame [14] using PCR with the following oligonucleotides primers: forward 5-catATGCCTCCAGCTGATGGAAC-3 and reverse 5-TCGCCAACGACTCCAATTATTAGCCAACGTCCAGACGTGGTTAGCAATTGGATACGCGGGcagctg-3