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These treatments decreased cell viability and increased both soluble and lipid reactive oxygen species (ROS) generation but did not affect mitochondrial ROS or mitochondrial mass
Postoperative / Rehabilitation Phase Rigid dressing / immediate post-operative prosthesis (IPOP): Reduces edema, accelerates rehabilitation timeline Stump shaping: Elastic bandaging, prosthetic shrinkers (HCPCS L8440/L8460) essential for preparing residual limb for prosthetic fitting Physical therapy: Strengthening, balance, gait training essential prior to and following prosthetic fitting Occupational therapy: Upper limb amputees ADL retraining, prosthetic use training Pain management: Multi-modal opioids (acute), gabapentin/pregabalin/duloxetine (neuropathic/phantom), mirror therapy, TENS, nerve blocks Wound care: Serial dressing changes, negative pressure wound therapy (NPWT) for complex wounds, hyperbaric oxygen (HBO) for ischemic or infected stumps Prosthetic Rehabilitation Prosthetic fitting: Initiated when residual limb volume stable (typically 48 weeks post-amputation for vascular
