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The protein sequences of the selected differentially expressed proteins were locally searched using the NCBI BLAST+ client software (ncbi-blast-2.2.28+-win32.exe) and InterProScan to find homologue sequences, after which gene ontology (GO) terms were mapped, and sequences were annotated using the software program Blast2GO

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Step two: calculate week 1-4 dose

This is the case for those using the drug to aid in weight loss, as the brain no longer seeks out food as a reward, but this can also impact the feeling of satisfaction or want for satisfaction throughout other parts of the body