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Notably, these circuits substantially overlap with those involved in the compulsive and reinforcing aspects of drug abuse

Injection Volume It is important to ensure that the administered volume of a substance causes the least discomfort possible to the mouse and does not exert a physiological change that might confound experimental findings.[1, 4, 7] Maximum dose volumes of the administered substance and recommended needle sizes vary subject to the chosen administration route (Table 1)

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this loss of stable ECD-ECD interaction was associated with increased motions of the RAMP-proximal loops of the CTR ECD (7-angstrom motion, measured at C of E123 ECD