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Additionally, eryptosis triggered by nanoscale materials can be associated with caspase-3 activation [452, 454] and calpain involvement [453, 458]

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Harvested yeast cells were mechanically lysed by beadmilling, using a micro-minibeadbeater (Biospec), in the presence of acid-washed glass beads (500 nm diameter) and lysis buffer (RLT) provided by the Qiagen RNeasy Mini Kit