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These data support our previous oral iron supplementation studies in iron-deficient, mostly non-anemic, subjects.178 In those studies, we assessed the magnitude and duration of the SHep increase after an oral iron dose and found that oral iron doses 60 mg significantly increased SHep at 24 h, which returned to baseline by 48 h.8 In a second study, we provided 14 oral iron doses of 60 mg to iron-depleted women either on 14 consecutive days or on alternate days over 28 days and showed that during the first 14 days of supplementation in both groups, SHep was higher in the consecutive day group than in the alternate day group, likely driven by the higher iron supplement frequency in the consecutive day group.17 Therefore, taken together, the available data suggest that in iron-deficient women with or without anemia, high oral iron doses acutely increase SHep and that this increase persists for 24 h but returns to baseline by 48 h

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doi: 10.1111/nure.12133 225