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Materials and Methods RAW264 Cells, GSH Depletion, and Treatment RAW264 cells were cultured in Roswell Park Memorial Institute medium 1640 with 2 mM l-glutamine (Sigma), 100 U/ml penicillin, 100 g/ml streptomycin sulfate (Invitrogen/Life Technologies), and 10% heat-inactivated FCS (Sigma)-omplete medium
[2] There is also a converse consideration that responsible modeling should note: a ghrelin-receptor agonist is, among other things, an appetite signal, and GHSR-1a activation has been associated with increased food intake in human work on related peptides
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