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Subsequently, Lineweaver-Burk [18] curves for the GR enzyme were obtained using three different concentrations of pyrimidine derivative compounds and five different concentrations of substrate (GSSG)

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Beyond altering the permeability of the BBB, acrylamide disrupts multiple lipid metabolic pathways, including cholesterol, arachidonic acid, sphingolipid, and phospholipid metabolism, mainly through the dysregulation of upstream PPAR signaling, which is heavily involved in lipid metabolism (74)

At higher concentrations, MMP2 and MMP9 decrease, preventing excessive collagen degradation