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Male C57BL/6 mice (10 weeks old) were anesthetized with isoflurane, and mesenteric arteries were then isolated and placed in ice-cold KrebsHenseleit solution, containing 112 mM NaCl, 5 mM KCl, 25 mM NaHCO 3 , 1 mM NaH 2 PO 4 , 0.5 mM MgCl 2 , 2.5 mM CaCl 2 , and 11.5 mM glucose

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While GLP-1 and GIP receptor activation primarily reduce energy intake through appetite suppression and delayed gastric emptying, glucagon receptor activation continues to drive energy expenditure through thermogenesis and hepatic fat oxidation regardless of whether caloric intake has already decreased