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Imaging of mitochondria by STED microscopy INS-1 832-3 cells were seeded in glass-bottom dishes (MatTek), labelled with 500 nM PKmito Orange FX (Spirochrome) for 30 minutes, washed 3 in PBS and incubated in complete media for 30 minutes to recover

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Such molecules can bind to proteins (which contain hundreds or even thousands of amino acids), altering these proteins function, and therefore serving as biochemical messengers

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