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Subsequently, the mixture was centrifuged at 12,000 g , 4 C for 5 min, and the supernatants were collected for LC-MS
203,204 Delivery of substrates to the 26S proteasome for degradation To avoid the deleterious accumulation of unfolded intermediates, the protein extracted from the ER needs to enter the 26S proteasome to enable effective degradation
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Recent studies using scintigraphy suggest that long-acting GLP-1 RAs may have fewer gastric effects than short-acting formulations due to the phenomenon of tachyphylaxis [20]
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