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The dosage and administration of GLP-1RAs@NPs, GLP-1RAs@NPs-WYRGRL or MNs/GLP-1RAs@NPs-WYRGRL were as follows: 50 l of 6 mg/ml GLP-1RAs was drawn to prepare GLP-1RAs@NPs, GLP-1RAs@NPs-WYRGRL or MNs/GLP-1RAs@NPs-WYRGRL, which was then injected or treated with MNs puncture into the sole of each rats foot