First, to a 1.5-mL Eppendorf tube were added 25 L of GSH (12 mM), 45 L of CDNB (3.0 mM), 50 L of GST (appropriate concentration), and 200 L of triethanolamineHCl buffer (20 mM, pH 6.5), and then, the mixed solution was incubated for 60 min at 25 C
They may also act directly to improve endothelial function, improve insulin sensitivity and enhance microvascular blood flow
Secondly, system X c , an antiporter of two catalytic subunits, is always utilized for transporting cystine into cells for GSH synthesis
Levels of AST, ALT, creatinine, and oxidative stress markers including TBARS, SOD, CAT, GPx, TAS, TOS, GSH, and GSSG, along with pro-inflammatory cytokines IL-1, IL-6, IL-8, IL-10, IL-17, and TNF-, were measured using ELISA kits