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Membranes were incubated with primary antibodies diluted in 5% milk 1X TBST overnight at 4C: anti-Actin (1:3000) (Sigma, A2066), anti-NRF2 (1:1000) (Cell Signaling Technology #12721) and anti-GSR (1:1000) (ThermoFisher, PA5-29945), followed by washing and incubation with HRP-conjugated anti-rabbit IgG (1:2000) (CST#7074) in 5% milk 1X TBST (22C, 1 h)

Brain Behav 10(10):e01773

In recent experiments the resulting electrochemically reduced graphene oxide showed a very high carbon to oxygen ratio and also electronic conductivity readings higher than that of silver (8500 S/m, compared to roughly 6300 S/m for silver)

Furthermore, when looking at the fluorescence levels relative to OD600, it is clear that fluorescence increases exponentially when glycine concentration increases