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Following incubation, beads were washed once with cold KPBS (pH=7.25) before being incubated in 200 l transport buffer (KPBS+10 mM HEPES + 0.5 mM EGTA) at room temperature containing indicated doses of [ 13 C 2 , 15 N]-GSH (Cambridge Isotope Laboratory, CNLM-6245-50) for the indicated times
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These are much less common now we have developed much more robust glove making systems
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