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Cholestatic injury is characterized by predominantly initial alkaline phosphatase level elevations that precede or are relatively more prominent than increases in the levels of serum aminotransferases

In a second study, the procedure above was repeated but with chymotrypsin as the protease (instead of trypsin) and with glutaryl-L-phenylalanine 4-methyl-7-coumarinylamide as the substrate, and a dose response curve with chenodeoxycholate at different concentrations was constructed

EMBO J.15, 37783786

Both procedures can be used to take a biopsy of intestinal tissue, which can reveal important information about the degree and extent of inflammation and help rule out other causes of symptoms