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For example, studies have shown that rutin exhibits lower scavenging activity compared to BHT, with both compounds demonstrating concentration-dependent scavenging of superoxide radicals, as indicated by their IC50 values (0.13 mg/mL for rutin and 0.16 mg/mL for BHT) (Yang et al., 2008a, b).Overall, the superoxide anion scavenging activity assay is a valuable tool for assessing the antioxidant potential of compounds, providing insights into their ability to mitigate oxidative stress and protect biological systems from ROS-induced damage(Carocho & Ferreira, 2013)

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this is known as the two-hit theory (3)