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Neutrophil to lymphocyte ratio: a new marker for predicting steatohepatitis and fibrosis in patients with nonalcoholic fatty liver disease

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Proteins were then separated via 8% SDS-PAGE (Servicebio, Wuhan, China), transferred to PVDF membranes, and blots were then blocked for 2 h at room temperature with 5% non-fat milk prior to incubation overnight with appropriate primary antibodies at 4C

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