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hCG directly stimulates Leydig cells to produce and release intratesticular testosterone by binding to the luteinizing hormone/choriogonadotropin receptor (Rizzuti et al., 2023)

For activity screening, we adapted a GSTO1 substrate assay that relies on the quantification of the reduction of S -(4-nitrophenacyl)glutathione (4-NPG), a GSTO1-specific substrate 17 , using recombinant human GSTO1 in a high-throughput screening format

von Holzen U, Pataer A, Bocangel D, Raju U, Vorburger SA, Liu Y, Lu X, Barber GN, Aggarwaal BB, Keyomarsi K, Hunt KK, Swisher SG

The liquid used to reconstitute lyophilized HCG is bacteriostatic water, which should come with your order