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INS-1 cells were grown in RPMI containing 10% (vol/vol) fetal calf serum, 10 mM HEPES, 2 mM L-glutamine, 1 mM sodium pyruvate, and 0.05 mM 2-mercaptoethanol at 37 C with 5% CO 2

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Study Objective and Why This Research Matters The central objective of this study was to determine whether supplementing older male rats with a combined daily dose of vitamin C and GSH could reduce age-associated oxidative stress and modulate downstream biochemical changes