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All expression plasmids were constructed using the Gibson Assembly Cloning Kit (New England Biolabs)

A hydrogen bond forms between one of the pyrrolidine fluorines and either Ser630 or Tyr631

Metabolic profiling of dried blood spots from patients with HS identified significant features that suggest a glycolytic disturbance, including a decrease in glyceraldehyde-3-phosphate, a glycolytic intermediate, and 2,3-bisphosphoglycerate, which is produced via conversion of 1,3-bisphosphoglycerate, another metabolite of glycolysis, in the LueberingRapoport glycolytic shunt and is responsible for enhancing oxygen dissociation from hemoglobin (Hb) (4)

antiaglomerante: talco