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In the early 1960s, researchers found that workers with chronic exposure to organophosphates, mainly in insecticides and after sheep dipping, experienced persistent central nervous system (CNS) changes (153)

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The autooxidation of pyrogallol under alkaline conditions generates superoxide, and the SOD-dependent inhibition of this process, tracked by a reduction in the rate of absorbance increase (at 420 nm or 440 nm), constitutes a well-established assay (Marklund and Marklund, 1974)

These dysfunctions may arise from impaired pyruvate dehydrogenase (PDH) function identified in muscle cells (390) and serum (184), as well as reduced plasma coenzyme Q10 (CoQ10) levels in ME/CFS blood and plasma (372, 391) that is inversely associated with fatigue severity (391)