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Preclinical studies using germ-free or antibiotic-treated animals have demonstrated impaired muscle mass, mitochondrial function, and endurance capacity, which are restored upon colonization with healthy microbiota or supplementation with specific probiotics such as Lactobacillus plantarum TWK10 and Bifidobacterium longum OLP- 01 [72,73,74,75]

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pH shift pH shift refers to the phenomenon whereby modulation of the solution pH alters the charge states of proteins or other molecules, thereby affecting their solubility, stability, and intermolecular interactions