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ILSI, Washington DC 1992:351 64
For the GFP tag knock-in construct, homology recombination templates were constructed with the following steps: (1) cloning a 2 kb genomic region of aptf-2 centered on the knock-in site into the pPD95.75 vector, (2) insertion of DNA encoding GFP and a linker of six amino acids (AGACCCAAGCTTGGTACC) between the last amino acid codon of aptf-2 and the stop codon, (3) the PAM site within the recombination template was mutated to prevent Cas9 cleavage
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