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Complex purification Cell pellet was thawed in 20 mM HEPES pH 7.4, 50 mM NaCl, 5 mM CaCls, 2 mM MgCl 2 supplemented with cOmplete Protease Inhibitor Cocktail tablets (Roche) and benzonase (Merk Millipore)

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The Dose-Response Relationship The trial enrolled adults with obesity (BMI 30) or overweight (BMI 27) with at least one weight-related health condition, excluding people with type 2 diabetes

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