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The demand for Se will increase after suffering environmental pressures, and supplementation is a necessary condition to meet the needs of fish growth ( 4.1 Harmful effects of selenium excess Se is an essential micronutrient required for fish, which plays an important role in the antioxidant system and also affects the lipid metabolism, sugar metabolism and amino acid metabolism of aquatic animals ( Acipenser transmontanus ), excessive Se in the diet leads to vacuolar degeneration of cells and necrosis of the liver ( 4.2 Harmful effects of Se deficiency The necessity and demand for dietary Se have been estimated in various fish (Zheng et al., 2018